Journal: International Journal of Molecular Sciences

Article Title: Analysis of the Expression and Activity of Cyclooxygenases COX-1 and COX-2 in THP-1 Monocytes and Macrophages Cultured with Xenogenic Collagen Matrices Biofunctionalized with the Injectable Platelet-Rich Fibrin

doi: 10.3390/ijms26094386

Figure Lengend Snippet: Imaging of COX-1 enzyme expression in THP-1 monocytes by fluorescence microscopy (I.) and representative Western blots (II.). Cells were cultured in RPMI medium with 10% FBS as the control condition for 48 h ( A ) and under the following conditions: MLCM ( B ), MLCM + iPRF ( C ), VSCM ( D ), VSCM + iPRF ( E ), BLCM ( F ), and BLCM + iPRF ( G ). The immunohistochemistry was performed using a specific primary antibody (mouse anti-COX-1 incubated overnight at 4 °C) and secondary antibodies conjugated with FITC (anti-mouse IgG-FITC, incubated for 45 min at room temperature). COX-1 expression is visible as green fluorescence, while cell nuclei were stained with DAPI (blue). Confocal microscopy was used for image analysis, employing filters 38 HE GFP for green fluorescence and 49 DAPI for blue fluorescence. Image analysis was performed with a confocal microscope using filters 38 HE GFP for green fluorescence and 49 DAPI for blue fluorescence. Arrows indicate areas of increased COX-1 expression compared with the control.

Article Snippet: The samples were analyzed using a confocal microscope (FV1000 confocal system with an IX81 inverted microscope, Olympus, Hamburg, Germany).

Techniques: Imaging, Expressing, Fluorescence, Microscopy, Western Blot, Cell Culture, Control, Immunohistochemistry, Incubation, Staining, Confocal Microscopy

Journal: International Journal of Molecular Sciences

Article Title: Analysis of the Expression and Activity of Cyclooxygenases COX-1 and COX-2 in THP-1 Monocytes and Macrophages Cultured with Xenogenic Collagen Matrices Biofunctionalized with the Injectable Platelet-Rich Fibrin

doi: 10.3390/ijms26094386

Figure Lengend Snippet: Imaging of COX-2 enzyme expression in THP-1 monocytes by fluorescence microscopy (I.) and representative Western blots (II.). Cells were cultured in RPMI medium with 10% FBS as the control condition for 48 h ( A ) and under the following conditions: MLCM ( B ), MLCM + iPRF ( C ), VSCM ( D ), VSCM + iPRF ( E ), BLCM ( F ), and BLCM + iPRF ( G ). Immunohistochemistry was performed using specific primary antibodies (mouse anti-COX-2, incubated overnight at 4 °C) and secondary antibodies conjugated with FITC (anti-mouse IgG-FITC, incubated for 45 min at room temperature). COX-2 expression is visible as green fluorescence, while cell nuclei were stained with DAPI (blue). A confocal microscope was used for image analysis, employing filters 38 HE GFP for green fluorescence and 49 DAPI for blue fluorescence. Arrows indicate areas of increased COX-2 expression compared with the control.

Article Snippet: The samples were analyzed using a confocal microscope (FV1000 confocal system with an IX81 inverted microscope, Olympus, Hamburg, Germany).

Techniques: Imaging, Expressing, Fluorescence, Microscopy, Western Blot, Cell Culture, Control, Immunohistochemistry, Incubation, Staining